ABSTRACT
<p><b>OBJECTIVE</b>Establishment of determination method of carbon disulfide in charcoal tube with low toxicity solvents desorption-gas chromatography.</p><p><b>METHODS</b>Four types of solvent with low toxicity are applied respectively as substitution of benzene to desorb the carbon disulfide in samples of charcoal tube. The signal strength and desorption efficiency of the detector are compared by using different solvents.</p><p><b>RESULTS</b>Chloroform has been considered as the best alternative solvent of benzene. Carbon disulfide has a good linearity (R = 0.9997) over the concentration of 0 ∼ 54.7 µg/ml, detection limit can reach 0.2 µg/ml. When the sampling volume is 3.0 L, the minimum detectable concentration is 0.07 mg/m(3).</p><p><b>CONCLUSION</b>With the use of chloroform, the health hazard to laboratory personnel and environment pollution as well as the costs of experiments are reduced.</p>
Subject(s)
Air Pollutants, Occupational , Carbon Disulfide , Chromatography, Gas , Methods , Solvents , WorkplaceABSTRACT
<p><b>OBJECTIVE</b>Establishment of determination method of 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine with HPLC.</p><p><b>METHODS</b>A volume of 0.5 ml hydrochloric acid (2 mol/L) and 0.5 ml pure water was added into 1 ml urine, and then extracted by 4 ml of diethyl ether by shaking for 2 min. Remove the water phase in a tube with plug and extract again, mix the two extraction diethyl ether together, take 4 ml by adding 2 ml borax-monopotassium phosphate buffer and shaking for 2 min to extract, then take the water phase to detect. A C(18) column and UV detector were used for separating and detecting. The wavelength was 273 nm, the flow rate was 1.0 ml/min, and the injection volume was 20 µl.</p><p><b>RESULTS</b>TTCA has a good linearity (r = 0.9995) over the concentration of1 1 ∼ 10 µg and the minimum detectable concentration of TTCA in urine was 0.1 µg/ml. The within-day precision (RSD) were 8.4%, 3.0% and 1.7%, the between-day precision (RSD) were 11%, 3.8%, 1.9%, respectively. The extraction recovery were between 80% ∼ 102%.</p><p><b>CONCLUSION</b>The method was accurate and sensitive to detect TTCA in urine.</p>
Subject(s)
Humans , Carbon Disulfide , Urine , Chromatography, High Pressure Liquid , Methods , Thiazolidines , UrineABSTRACT
<p><b>OBJECTIVE</b>To study the biological exposure index of carbon disulfide in China.</p><p><b>METHODS</b>High-performance liquid chromatography (HPLC) was used to detect the levels of 2-thiothiazolidine-4-carboxylic acid (TTCA) in the urine of the workers after working shift end, Gas chromatography was used to detect the concentrations of the carbon disulfide in the workplace air. The relationship between the urine TTCA levels and the concentrations of the carbon disulfide was analyzed, the biological exposure index and judgement result from PC-TWA were compared.</p><p><b>RESULTS</b>The levels of TTCA in urine of workers occupationally exposed to carbon disulfide were closely and positively related with the concentrations of the carbon disulfide in the workplace air. The regression equation was Y = 0.265X - 0.165, The biological exposure index of carbon disulfide were calculated by regression equation according to occupational exposure limits of carbon disulfide in China.</p><p><b>CONCLUSION</b>The biological exposure index of CS(2) in China might be revised for 1.2 mg/g Cr.</p>